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The culture was supplemented with ExpiCHOFeed and ExpiFectamine CHO Enhancer and maintained for 3 days in a 37 C incubator with a humidified atmosphere of 8% CO 2 in air on an orbital shaker

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When the acute inflammatory response is not resolved, it can contribute to organ pathology and amplify many widely occurring chronic inflammatory clinical phenotypes, including neurodegenerative diseases, metabolic syndrome, asthma, allergy, diabetes, inflammatory processes of aging, arthritis, cancers, organ fibrosis, and cardiovascular and periodontal diseases [331]